ActionStep
Fill a syringe with approximately 10 ml of the first pH standard buffer. Connect the syringe
to the Luer connector of pH valve port Cal, and inject the buffer.
4
When the Current value is stable, click the Calibrate button.5
Wash the pH flow cell by injecting water into pH valve port Cal using a new syringe.6
Enter the pH of the second pH standard buffer in the pH for buffer 2 field.7
Repeat steps 4-6 using the second pH standard buffer.8
Result: The calibration date and time are displayed in the dialog, along with values for
Calibrated electrode slope and Asymmetry potential at pH 7.
Is the Calibrated electrode slope ≥ 80% and the Asymmetry potential at pH 7 inside
the interval ± 60 mV?
9
•
If Yes: Click the Close button to switch the pH valve back to the default position and
to close the Calibration dialog.
•
If No: Clean the pH electrode and repeat the calibration procedure. If this does not
help, replace the electrode.
304 ÄKTA pure User Manual 29119969 AB
7 Maintenance
7.7 Calibration procedures
7.7.1 Calibrate the pH monitor
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