ActionStep
In the Run Data pane of System Control, read the current Cond temp.
Tip:
If Cond temp is not showing, click the Customize icon. In the Customize dialog, under the
Run Data Groups tab, select to view Cond temp.
9
In the Calibration dialog, enter the theoretical conductivity value at the current conduc-
tivity temperature in the Enter theoretical conductivity value input field.
10
•
If a certified conductivity standard solution is used, use the supplied theoretical con-
ductivity value.
•
If a manually prepared 1.00 M NaCl calibration solution is used, see the graph for
conductivity value at the current temperature on page 311.
In the Calibration dialog, click Calibrate.11
Result: The new conductivity cell constant is displayed in the Conductivity cell 1 con-
stant/cm box. The new constant should normally be 40 ± 10 cm
-1
. The date and time for
the calibration are also displayed.
In the System Control toolbar, click the End icon to end the run.12
In the System Settings dialog, select Conductivity:Cond temp compensation and set
the Compensation factor back to desired value, default 2.0%. Click OK.
Note:
When using NaCl, the optimal compensation factor is 2.1%.
13
310 ÄKTA pure User Manual 29119969 AB
7 Maintenance
7.7 Calibration procedures
7.7.3 Calibrate the Conductivity monitor
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