GE AKTApure - Page 343

ActionStep
•
For a system pump, in the Manual instructions dialog:
-
Select Flow path:Column valve, and select the position of the tubing connected
to the Column valve, in this example Position 1. Click Insert.
-
Select Flow path:Inlet A (for System pump A) or Flow path:Inlet B (for System pump
B) and select a Position, in this example A1 or B1. Click Insert.
-
Select Pumps:Gradient and set Target to 0% B (for System pump A) or 100% B
(for System pump B).
-
For pump P9: Select Pumps:System flow and set the Flow rate to 5.0 ml/min. Click
Insert.
-
For pump P9H: Select Pumps:System flow and set the Flow rate to 25.0 ml/min.
Click Insert.
-
Click Execute.
Result: A system flow starts.
•
For a sample pump, in the Manual instructions dialog:
-
Select Flow path:Column valve, and select the position of the tubing connected
to the Column valve, in this example Position 1. Click Insert.
-
Select Flow path:Sample inlet and select a Position, in this example S1. Click Insert.
-
Select Flow path:Injection valve and select Direct inject from the Position drop-
down list. Click Insert.
-
Select Pumps and Pressures:Sample flow and set the Flow rate to 25.0 ml/min.
Click Insert.
-
Click Execute.
Result: A sample flow starts.
4
Run the flow for 2 hours.5
Discard the used buffer.6
ÄKTA pure User Manual 29119969 AB 343
7 Maintenance
7.8 Replacement procedures
7.8.10 Replace pump piston seals of Pump P9 or P9H