•
Does the positioning of the columns correspond to the selections made in the method? For more
details, see Section 5.5 Connect a column, on page 173.
Purification checks
•
Have all columns been cleaned and prepared according to the column recommendations?
•
Have the samples been adjusted to binding buffer conditions?
•
Have the samples been clarified by centrifugation and/or filtration prior to sample loading?
•
Are the correct buffers used for the chosen columns and proteins?
•
Check buffers for precipitations. Adjust to room temperature.
•
Are the chosen columns suitable for the chosen target proteins?
•
Do the buffers have correct pH? The pH of some buffers changes with the temperature.
•
Are the UV-wavelengths used by the method appropriate with respect to used buffers and proteins?
For more details see the method handbooks available from GE.
ÄKTA pure User Manual 29119969 AB 359
8 Troubleshooting
8.2 Troubleshooting: General Checklist
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