Possible cause and actionProblem
•
Calibration time out
Check the connections between pH electrode and pH monitor.
Regenerate the pH electrode. Place the electrode in deionized
water for 30 minutes followed by 30 minutes in a buffer with pH
4.
If persistent, replace the pH electrode. See Section 7.5.2 Replace
the pH electrode, on page 275.
•
Bad pH electrode
Regenerate the pH electrode. Place the electrode in deionized
water for 30 minutes followed by 30 minutes in a buffer with pH
4.
Clean the pH electrode. See Section 7.6.7 Clean the pH electrode,
on page 293.
If persistent, replace the pH electrode. See Section 7.5.2 Replace
the pH electrode, on page 275.
•
Wrong mixer size for the used flow rate
Use the recommended mixer size for the used flow rate. See
Unstable pH signal
•
Decreasing salt concentration in the electrode membrane due
to osmosis to buffer
Regenerate the pH electrode. Place the electrode in deionized
water for 30 minutes followed by 30 minutes in a buffer with pH
4.
Drift of pH signal when the
pH electrode has been re-
moved from storage solu-
tion
•
The temperature compensation of the pH monitor is turned
off
Contact Service.
Temperature reading error
•
Waste tubing is twisted or blocked
Untwist the tubing.
Perform System CIP to clean waste tubing. See Section 7.6.2
Perform System CIP, on page 278.
Change the tubing.
It is not possible to inject
calibration solution
ÄKTA pure User Manual 29119969 AB 371
8 Troubleshooting
8.3 Troubleshooting: Monitors
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