Possible cause and actionProblem
•
Air bubbles are passing through or are trapped in the pump
Check that there is a sufficient volume of buffer present in the
flasks.
Check all connections for leaks.
Check pump pressure curves. See Examples of pump pressure
curves, on page 390 for examples of pump pressure curves.
Purge the pumps, see Section 5.4.1 System pumps, on page 162
or Section 5.4.2 Sample pump, on page 168
•
The check valve does not function correctly
Remove any solids in the valves by cleaning the check valves
according to the instructions in Section 7.6.8 Clean the pump
head check valves, on page 296.
•
Piston seal is leaking
Replace the piston seal of a system pump according to the in-
structions in Section 7.8.10 Replace pump piston seals of Pump
P9 or P9H, on page 335. Replace the piston seal of the sample
pump according to the instructions in Section 7.8.11 Replace
pump piston seals of Pump P9-S, on page 344
•
Blockage of flow path
Use the predefined method Prepare System to flush through to
clear blockage.
If necessary, replace tubing. See Section 7.8.1 Replace tubing and
connectors, on page 317.
Check the mixer inline filter. It can be clogged if unfiltered buffers
or samples are applied. See Section 7.3.2 Replace the inline filter,
on page 266 for instructions how to replace the mixer inline filter.
Check the inlet filters. They can be clogged if unfiltered buffers
or samples are applied. To replace the filters, see Section 7.8.8
Replace the inlet filters, on page 331.
Check the column. It can be clogged if unfiltered buffers or
samples are applied. To clean a column, see Section 7.6.3 Perform
Column CIP, on page 284.
Irregular pressure curves
374 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.3 Troubleshooting: Monitors
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