Included phasesPrinciplePredefined purification
method
After equilibration and sample application, neg-
atively charged proteins are adsorbed to the
column ligand. After a wash, to remove unbound
sample, elution is performed using a gradient of
increasing salt concentration (of e.g. NaCl). Final-
ly, the column is washed and re-equilibrated
with start buffer.
Anion Exchange Chromatogra-
phy (AIEX)
After equilibration and sample application, posi-
tively charged proteins are adsorbed to the col-
umn ligand. After a wash, to remove unbound
sample, elution is performed using a gradient of
increasing salt concentration (of e.g. NaCl). Final-
ly, the column is washed and re-equilibrated
with start buffer.
Cation Exchange Chromatog-
raphy (CIEX)
After equilibration and sample application, elu-
tion is performed using a pH gradient. The pro-
teins separate and elute according to their iso-
electric points. Finally, the column is re-equilibrat-
ed.
Chromatofocusing (CF)
ÄKTA pure User Manual 29119969 AB 441
9 Reference information
9.7 Predefined methods and phases
9.7.1 Predefined purification methods
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