Standard delay volumes
The table below lists the delay volumes for standard configurations using the available tubing kits with
and without a pH valve and using tubing of standard length from the outlet valve to the fraction col-
lector. The values are based on the flow cells for UV monitor U9-M. When using U9-L, add 9 µl for the
2 mm flow cell and 4 µl for the 5 mm flow cell. The values for delay volume Monitor to frac F9-C assume
that the internal tubing of Fraction collector F9-C has the same inner diameter as the tubing kit.
Delay volumes ÄKTA pure 25
Delay volume UV monitor to point of collection of eluentDelay vol-
ume pH
valve re-
strictor
pH
valve
V9-pH
Tubing kit
Delay volume
Monitor to frac
F9-C
Delay volume
Monitor to frac
F9-R
Delay volume
Monitor to outlet
valve
214 µl86 µl66 µlN/ANo0.25
225 µl96 µl77 µl25 µlYes0.25
435 µl205 µl125 µlN/ANo0.5 (stan-
dard)
462 µl231 µl152 µl48 µlYes0.5 (stan-
dard)
803 µl400 µl223 µlN/ANo0.75
857 µl454 µl277 µl88 µlYes0.75
1319 µl675 µl361 µlN/ANo1.0
1410 µl766 µl452 µl143 µlYes1.0
Delay volumes ÄKTA pure 150
Delay volume UV monitor to point of collection of eluentDelay vol-
ume pH
valve re-
strictor
pH
valve
V9H-pH
Tubing kit
Delay volume
Monitor to frac
F9-C
Delay volume
Monitor to frac
F9-R
Delay volume
Monitor to outlet
valve
508 µl278 µl198 µlN/ANo0.5
555 µl324 µl245 µl60 µlYes0.5
876 µl473 µl296 µlN/ANo0.75 (stan-
dard)
950 µl547 µl370 µl100 µlYes0.75 (stan-
dard)
1392 µl748 µl434 µlN/ANo1.0
1503 µl859 µl545 µl155 µlYes1.0
496 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.13 Delay volumes
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