ActionStep
Start the pump at a flow rate of 1 ml/min and inject the acetone solution.8
Note the volume from the point of injection to the appearance of the peak in the chro-
matogram. This volume is volume measurement V2.
9
To obtain the delay volume:10
•
Subtract V1 from V2 and
•
add half of the volume of the UV flow cell.
Use of different monitors for peak fractionation
in the same method
If different monitors or detectors are used for peak fractionation in different parts of the same method,
the delay volumes have to be set as method instructions for each of the method parts. For example,
both an external fluorescence detector and the UV monitor module can be used for peak fractionation.
498 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.13 Delay volumes
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