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Quenching Protocol – Hendrickson – Klughammer
Lake Model:
The OS1p can be ordered with various quenching protocols, but the advanced version
comes with the Hendrickson – Klughammer lake model protocol as standard. Other
options include, Dave Kramer lake model protocol, puddle model protocol, and
quenching relaxation protocol that may be used with either the Hendrickson or puddle
model protocols. For an in depth discussion of the value and differences between the
protocols go to page quenching section in the first chapter. The Hendrickson –
Klughammer protocol was chosen because NPQ has been resurrected by Klughammer for
the lake model of antennae – reaction center interaction using the Hendrickson equations.
There are very few reference papers available that use the Kramer lake model at this
time, however, NPQ is well understood by most, and there is a wealth of reference
literature available for the many uses of NPQ.
One can choose Kramer protocol, Hendrickson – Klughammer protocol, or puddle model
protocol at the time of purchase. If no selection is made, then the Hendrickson –
Klughammer lake model protocol will be supplied. Other protocols may be added for an
additional price, or they may be added at a later date for an additional price.
The OS5p offers all of these protocols as standard.
Hendrickson - simplified equations
Y(II) = (Fm’ -Fs)/Fm’ or )Fm’/ Fm’
Y(NO) = Fs/Fm or F/Fm
Y(NPQ) =( Fs/Fm’)-Y(NO) or (F/Fm’) - Y(NO)
Klughammer -NPQ was resurrected from the puddle model for the lake model
using Hendrickson’s equations.
NPQ= Y(NPQ)/Y(NO) or NPQ = (Fm
-Fm’)/Fm’
Using the Hendrickson Klughammer protocol.
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