2
CHAPTER 1 • INTRODUCTION....................................................................................................................... 6
OVERVIEW...........................................................................................................................................................6
WELCOME ! .........................................................................................................................................................7
LIST OF EQUIPMENT.............................................................................................................................................7
GETTING STARTED...............................................................................................................................................7
CHLOROPHYLL FLUORESCENCE...........................................................................................................................8
DEFINITIONS:.....................................................................................................................................................11
QUANTUM PHOTOSYNTHETIC YIELD OF PSII – AN IN DEPTH DISCUSSION OF ITS VALUE AND LIMITATIONS. .......16
QUENCHING MEASUREMENTS, AN OVERVIEW ...................................................................................................21
Quenching equations: ..................................................................................................................................24
Definitions - lake model parameters............................................................................................................25
Definitions - puddle model parameters........................................................................................................26
Understanding the quenching mode trace ...................................................................................................28
More helpful hints for setting test variable in quenching protocols. ...........................................................33
DARK ADAPTATION – HOW LONG IS LONG ENOUGH? .......................................................................................35
RELATIVE ELECTRON TRANSPORT RATE ...........................................................................................................37
BIBLIOGRAPHY ..................................................................................................................................................40
CHAPTER 2 • THE OS1P.................................................................................................................................. 52
INTRODUCTION ..................................................................................................................................................52
KEY FEATURES ..................................................................................................................................................53
PHYSICAL FEATURES PANEL PHOTOGRAPHS ...................................................................................................54
HARDWARE OVERVIEW AND MEASUREMENT PRINCIPLES .................................................................................56
LIGHT SOURCES .................................................................................................................................................57
Modulated light source ................................................................................................................................57
Saturation pulse light source .......................................................................................................................57
The LED actinic light source .......................................................................................................................58
The far-red light source ..............................................................................................................................58
THE FIBER OPTIC LIGHT GUIDE .........................................................................................................................58
ELECTRONICS ....................................................................................................................................................59
HOUSING AND CARRYING CASE.........................................................................................................................59
CHAPTER 3 • OPERATING THE OS1P......................................................................................................... 60
INTRODUCTION ..................................................................................................................................................60
INTRODUCTION TO RUNNING TESTS....................................................................................................................60
Set up menu..................................................................................................................................................61
Adjust screen touch sensitivity .....................................................................................................................62
Touch Panel Calibrate.................................................................................................................................62
Test menu .....................................................................................................................................................63
FV/FM PROTOCOL (OR FV/M ON THE SCREEN)..................................................................................................64
Cookbook check list before making Fv/Fm measurements ..........................................................................65
Running the Fv/Fm test................................................................................................................................67
Drill down view of Fv/Fm measuring screens .............................................................................................68
Changing Fv/Fm measuring parameters -...................................................................................................69
Setting the modulation light source intensity...............................................................................................69
New -automated modulation light set up .....................................................................................................69
Far red light.................................................................................................................................................70
Setting the saturation pulse intensity ...........................................................................................................70
Setting saturation pulse duration.................................................................................................................70
Entering a note with a measurement............................................................................................................72