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Fs also known as F’ is the fluorescence level created by the actinic light . Initially the value is
high and then decreases over time to steady state values due to the initiation of electron
transport, carboxilation, and nonphotochemical quenching. Fs has also been used to designate
steady state F’ conditions.
Fms – also known as Fm’ is the saturation pulse value that is not dark-adapted. They are at a
lowered values due to NPQ or non-photochemical quenching. When this parameter has
reached steady state, it is used to calculate photosynthetic Yield - Y(II) or )F/Fm’ along with
Fs. Fms at steady state is also used to calculate qN, NPQ, qP, qL, Y(NPQ), Y(NO), qE, qT,
and qI.
Fo is minimal fluorescence after dark adaptation. It is measured with a modulated light
intensity too dim to drive photosynthesis and yet bright enough to detect “pre-photosynthetic”
antennae fluorescence.
Fod – also known as Fo’, is the minimal value after the actinic light has been turned off and
after a far red light is turned on for several seconds. It represents Fo with non-photochemical
quenching. It may also be described as minimum chlorophyll fluorescence yield with
maximal opening of all PS II reaction center traps in a light-acclimated state.
Ft – is the current instantaneous fluorescent signal shown on the fluorometer measuring
screen. It is used to set the modulated light source intensity. See setting the modulated light
source intensity.
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