Thermo NanoDrop One User Manual

3
Learning Center
Measure a Sample Using a Cuvette
Thermo Scientific NanoDrop One User Guide 201
Best practices for cuvette measurements
To measure a sample using a cuvette
• The instrument arm can be up or
down for cuvette measurements.
• Use 10 mm, 5 mm, 2 mm or 1 mm
cuvettes up to 48 mm tall.
• Clean and dry cuvette after each
measurement.
• Use cuvettes that are free of scratches
and avoid fingerprints which may
affect results.
• Use quartz cuvettes or UV-grade
plastic cuvettes to measure samples
with analysis wavelengths in the
UV range (<340 nm).
• Micro, semi-micro, and ultra-micro
cuvettes should be masked.
• Fill cuvettes with enough blanking or
sample solution to cover instrument
optical path (2 mm sample beam is
8.5 mm above cuvette bottom).
• Lift instrument arm and make sure
cuvette holder is free of debris.
• When inserting quartz or masked
plastic cuvettes, align cuvette light path
with instrument light path.
Instrument light path
Cuvette holder
NOTICE
• To prevent damage from spills, keep containers of liquids away from the instrument.
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)