Zeiss Axio Vert.A1 - Page 4

Carl Zeiss Copyright / Trademarks Axio Vert.A1
4 431030-7044-001 05/2012
3.1.11 Attaching Illuminators for Reflected Light ....................................................................... 52
3.1.12 Adjusting the HAL 100 Illuminator .................................................................................. 58
3.1.13 Changing the HAL 100 Halogen Lamp ............................................................................ 59
3.2 Connecting the Microscope to the Power Supply System, Switching the
Microscope on and Setting It up ................................................................................ 60
3.3 Mounting Optional Components ............................................................................... 61
3.3.1 Changing Diaphragms in the Condenser with Modulator Disk ......................................... 61
3.3.2 Changing Diaphragms in Sliders ..................................................................................... 63
3.3.3 Changing the Filter Set in the FL P&C Reflector Module.................................................. 64
3.3.4 Changing the Beam Splitter in the FL P&C Reflector Module ........................................... 65
3.3.5 Changing the Beam-Splitting Mirror in the Intermediate Phototube ................................. 67
3.3.6 Mounting the Aqua Stop ............................................................................................... 68
3.3.7 Changing LED Modules in the Axio Vert.A1 FL-LED Stand ............................................... 69
3.3.8 Mounting the Carrier for Transmitted Light Illumination onto the
Axio Vert.A1 MAT Stand ................................................................................................ 70
4 OPERATION ....................................................................................................... 71
4.1 Use of LD Objectives ................................................................................................... 71
4.2 Use of Korr Objectives ................................................................................................ 71
4.3 Use of Immersion Objectives ...................................................................................... 72
4.4 Possible Condenser, Objective and Diffusion Disk Combinations ............................ 72
4.5 Use of Objectives ........................................................................................................ 73
4.6 ECO Power-Saving Function ....................................................................................... 73
4.7 Height Stop for the Focusing Drive ............................................................................ 74
4.8 Operating Functions on the Axio Vert.A1 FL-LED Stand ........................................... 74
4.9 Light Manager Function on the Axio Vert.A1 MAT Stand ........................................ 75
4.10 USB Interface on the Axio Vert.A1 MAT Stand ......................................................... 75
4.11 Illumination and Contrast Techniques in Transmitted Light ..................................... 76
4.11.1 Transmitted Light Bright Field ......................................................................................... 76
4.11.2 Transmitted Light Phase Contrast ................................................................................... 77
4.11.3 Transmitted Light VAREL Contrast .................................................................................. 79
4.11.4 Transmitted Light Differential Interference Contrast (DIC) ................................................ 80
4.11.5 Transmitted Light PlasDIC ............................................................................................... 82
4.11.6 Transmitted Light iHMC ................................................................................................. 83
4.11.7 Transmitted Light Polarization Contrast ......................................................................... 86
4.12 Illumination and Contrast Techniques in Reflected Light ......................................... 87
4.12.1 Reflected Light Bright Field ............................................................................................. 87
4.12.2 Reflected Light Dark Field ............................................................................................... 89
4.12.3 Reflected Light DIC ........................................................................................................ 90
4.12.4 Reflected Light Polarization ............................................................................................ 92
4.12.5 Reflected Light Fluorescence .......................................................................................... 94
4.13 Micromanipulators ...................................................................................................... 96