OPERATION
Carl Zeiss Use of Immersion Objectives Axio Vert.A1
72 431030-7044-001 05/2012
4.3 Use of Immersion Objectives
In immersion objectives, the air between the cover slip and the objective is replaced by a liquid, called
immersion oil.
Put a small bubble-free drop of Immersol 518 N
(for transmitted light applications) or 518 F (for
fluorescence) on the front lens of the objective. Then place the cultivation vessel or the specimen, with
the cover slip facing the objective, on the specimen stage or into the mounting frame.
Next, carefully approach and focus the objective.
Do not forget to remove the immersion oil with a soft cloth (which may be wetted with benzine) from
the objective after each experiment.
Excessive amounts of immersion oil may get into the mechanical system of inverted microscopes, where
the oil could adversely affect their functionality.
For cleaning suggestions, please refer to the brochure entitled "The Clean Microscope."
4.4 Possible Condenser, Objective and Diffusion Disk Combinations
The following combinations are recommended:
Condensers Objectives Diffusion Disks
Condenser 0.3 Objective 1.25x Diffusion disk 424228-9050-000
in the slider of the condenser
Objectives 2.5x to 40x *) -
Condenser 0.4 Objective 2.5x Diffusion disk 424228-9040-000
in the slider or the modulator
disk of the condenser
Objectives 5x to 63x *) -
Condenser 0.55 Objectives 5x to 100x -
*) Use with objective 100x possible, but higher contrast and thus lower resolution due to difference in aperture
CAUTION
Be certain to heed the safety comments on Page 10 relating to the use of immersion oil.
To Next Page
Loading...
