OPERATION
Carl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A1
78 431030-7044-001 05/2012
• Use the relevant centering screws (Fig. 4-3/2) on the slider to line up the phase ring and the phase
stop. Make sure that the gray phase ring of the objectives fully covers the bright phase stop
(Fig. 4-4/C).
Should the setting range of the centering screws prove insufficient, please check whether
the condenser sits on the front stop and whether the slider is locked into position. A plane-
parallel specimen is required if an exact image of the phase stop is to be obtained.
Use two SW 1.5 Allen wrenches to center the phase stop for the condenser with modulator
disk. The holes for accessing the centering screws are
located to the left and right on the
condenser.
• Then replace the auxiliary microscope by the eyepiece.
Due to the fluid meniscus, working with Ph in the peripheral areas of the cavity can prove
problematic, especially when it comes to multiwell dishes. If this is unavoidable, the Ph stop
may be readjusted on the condenser. Alternatively, you can utilize a contrast which is less
sensitive in this respect, such as PlasDIC.
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