Zeiss Axio Vert.A1 - Page 91

OPERATION
Axio Vert.A1
Illumination and Contrast Techniques in Reflected Light Carl Zeiss
05/2012 431030-7044-001 91
Setting C-DIC
• Set the microscope up for reflected light bright field.
• Rotate the C-DIC P&C reflector module into the light path.
• Insert C-DIC slider 6x20 (Fig. 4-15/1) into the slot.
• Put on the specimen, focus and turn the adjustment wheel on C-DIC slider 6x20, so that the structure
of interest is visible with maximum contrast.
• You can create additional contrast effects by turning the adjustment wheel on the C-DIC slider.
Additional Comment
When using the differential interference contrast or the differential interference contrast in circularly
polarized light, the contrast develops through a pseudo-relief in the specimen. When it comes to linear
structures, the contrast depends, therefore, on whether those structures are oriented in "light-shadow"
direction (very little contrast) or vertically to it (maximum contrast).
When you employ C-DIC slider 6x20, you can align the structures vertically to the "light-shadow"
direction and thus set maximum contrast by turning the adjustment wheel of the C-DIC slider.