5 First Operating Steps | 5.7 Working with Specific Specimen Types ZEISS
8. Use the stub tool to place the plain stub on the
Peltier-coolstage specimen holder. Ensure that
the stub is positioned horizontally (is not tilted)
to ensure good contact to the cooling area.
INFO: For wet imaging of hydrated specimens,
it is essential to maintain a constant cooling
temperature on the stub.
9. Use a hex key 1.5mm to carefully tighten the
clamp screw.
10. Re-fit the BSD if required.
11. Carefully close the chamber door without trap-
ping the pipe work of the Peltier coolstage.
104 Instruction Manual ZEISS EVO | en-US | Rev. 10 | 354706-0780-006