HA-8180V 3-34
Chapter 3 Auxiliary Operations
3.7.6 Testing HbA1C Reproducibility (Whole Blood Sample)
Run a reproducibility test following the instructions described below if the reproducibility of HbA1C measurement
results seems to have been reduced in anemia or non-anemia whole blood sample measurements. This test repeatedly
measures the same whole blood sample and displays statistical information (average, R, S.D. and C.V.) from those
results. Since all measurements are performed using sample from a single sample tube, there is no need to divide the
sample into multiple sample tubes before setting them.
Prepare: For whole blood sample test
Whole blood sample, sample tube (
x 1) ,
normal rack (or start rack for loop transportation) and protective gloves
For anemia sample test
Anemia sample, sample tube (
x 1), anemia rack and protective gloves
1 Prepare sample.
a On the standby screen, check whether the measurement mode is set to the mode you want to test.
• To change the mode, see “2.4. Measurement Mode Selection (Variant or Fast)” on page 2-14.
b On the standby screen, select , <8 Instrument diagnosis menu>, <5 HbA1c reproducibility test menu> and
<1 Whole sample measurement> in that order.
• The message “Load samples onto sampler.” will appear.
c Prepare sample in a sample tube.
• Make sure the sample tube is capped.
Wear protective gloves to prevent exposure to pathogenic microbes.
Discard used samples and protective gloves in accordance with local regulations for
biohazardous waste.
Performing measurements with uncapped tubes may cause sample to splatter inside the
instrument while spinning, possibly jeopardizing subsequent measurements. It may also cause
infection to the user or other individuals by pathogenic microbes.
The required sample volume (whole blood) is shown below. Add a sufficient volume of sample to the sample
tube to perform the set number of measurements.
(Required sample volume) = 14 μL x (Set number of measurements) + 1 mL
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