Replacing the waste container cap 40
Changing the sheath filter 41
Changing the Bal seal 42
Changing the sample tube O-ring 44
Cleaning or replacing the sheath gasket 45
5 SPECTRAL FUNCTIONS 47
Spectral function overview 48
Autofluorescence 54
Spectral unmixing software considerations 55
Setting up a new spectral experiment 57
Creating a Spectral Unmixing matrix 59
Recording and analyzing spectral data 62
Repeating a spectral experiment 65
6 OPTIMIZING CYTOMETER SETTINGS 67
Cytometer settings workflow 68
Verifying the configuration and user preferences 69
Running a performance check 71
Setting up a compensation experiment 74
Creating application settings 77
Recording compensation controls 78
Calculating compensation 81
7 RECORDING AND ANALYZING DATA 83
Data recording and analysis workflow 84
Preparing the workspace 84
Recording data 85
Analyzing data 87
Reusing an analysis 91
8 MANUAL SETTINGS 93
About laser delay 94
Optimizing laser delay 95
Adjusting area scaling 97
9 TROUBLESHOOTING 103
Cytometer troubleshooting 104
Electronics troubleshooting 109
10 SUPPLIES AND CONSUMABLES 111
Ordering information 112
Beads 112
Reagents 113
4 BD FACSymphony™ A5 SE User's Guide
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