BD LSR II - Page 119

Chapter 7: Troubleshooting 119
Low event rate Threshold level too high Lower the threshold level. Refer to
the BD FACSDiva Software
Reference Manual
for
instructions.
PMT voltage for threshold
parameter set too low
Set the PMT voltage higher for the
threshold parameter. Refer to the
BD FACSDiva Software
Reference Manual
for
instructions.
Sample not adequately mixed Mix the sample to suspend cells.
Sample too dilute Concentrate the sample. If the
flow rate setting is not critical to
the application, set the flow rate
switch to MED or HI.
Sample injection tube clogged Remove the sample tube to allow
backflushing; then run a tube of
warm DI water for 20 minutes.
If the event rate is still erratic,
clean the sample injection tube.
See Daily Cleaning and Shutdown
on page 100.
Erratic event rate Sample tube cracked Replace the sample tube.
Bal seal worn Replace the Bal seal. See Changing
the Bal Seal on page 110.
Sample injection tube clogged Remove the sample tube to allow
backflushing; then run a tube of
warm DI water for 20 minutes.
If the event rate is still erratic,
clean the sample injection tube.
See Daily Cleaning and Shutdown
on page 100.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
LSR2.book Page 119 Tuesday, April 25, 2006 3:34 PM