118 BD LSR II User’s Guide
High event rate Air bubble in sheath filter or
flow cell
Remove the air bubble. See
Removing Air Bubbles on
page 47.
Threshold level too low Increase the threshold level. Refer
to the
BD FACSDiva Software
Reference Manual
for
instructions.
PMT voltage for threshold
parameter set too high
Set the PMT voltage lower for the
threshold parameter. Refer to the
BD FACSDiva Software
Reference Manual
for
instructions.
Sample too concentrated Dilute the sample.
Sample flow rate set on HI Set the sample flow rate to MED
or LO.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
LSR2.book Page 118 Tuesday, April 25, 2006 3:34 PM
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