Chapter 7: Troubleshooting 117
No events in acquisition
display and orange RUN
button (continued)
Sheath container empty Fill the sheath container.
Air in sheath filter Purge the filter. See Removing Air
Bubbles on page 47.
No fluorescent signal Incorrect fluorochrome
assignment
Make sure the instrument
configuration in the software
matches the optical filters in the
instrument.
Wrong filter installed Make sure the appropriate filter is
installed for each fluorochrome.
See Changing Optical Filters or
Mirrors on page 42.
Laser not functioning Verify laser malfunction by
changing the threshold to an
alternative laser while running
appropriate QC particles. If not
successful, contact
BD Biosciences.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
LSR2.book Page 117 Tuesday, April 25, 2006 3:34 PM
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