BD LSR II - Page 116

116 BD LSR II User’s Guide
No events in acquisition
display and green RUN
button (continued)
Laser not warmed up Wait the recommended amount of
time for the laser to warm up.
• 30 min for the 488-nm (blue)
• 30 min for the 355-nm (UV)
• 15 min for the 405-nm (violet)
• 20 min for the 633-nm (red)
Laser delay set incorrectly Adjust the laser delay settings.
See Setting Laser Delay on
page 193.
Laser not functioning Verify malfunction by changing
the threshold to an alternative
laser while running appropriate
QC particles. If not successful,
contact BD Biosciences.
No events in acquisition
display and orange RUN
button
RUN not activated Press the RUN button.
Sample tube not installed or
not properly seated
Install the sample tube correctly
on the SIP.
Sample tube cracked Replace the sample tube.
Sheath container not
pressurized
• Ensure that the sheath
container lid and all connectors
are securely seated.
• Inspect the O-ring and replace
if necessary. See Changing the
Sample Tube O-Ring on
page 112.
Bal seal worn Replace the Bal seal. See Changing
the Bal Seal on page 110.
Air leak at sheath container Ensure that the sheath container
lid and all connectors are securely
seated.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
LSR2.book Page 116 Tuesday, April 25, 2006 3:34 PM