122 BD LSR II User’s Guide
Poor QC results Air bubble or debris in flow
cell
Prime the fluidics system. See
Priming the Fluidics on page 49.
Old or contaminated QC
particles
Make new QC samples and
perform the quality control
procedure again.
Sample not diluted in same
fluid as sheath fluid
Dilute the sample in the same fluid
as you are using for sheath. If you
are running BD CaliBRITE™
beads, dilute them in
BD FACSFlow solution and use
BD FACSFlow solution for sheath
fluid.
Laser not warmed up Wait the recommended amount of
time for the laser to warm up.
• 30 min for the 488-nm (blue)
• 30 min for the 355-nm (UV)
• 15 min for the 405-nm (violet)
• 20 min for the 633-nm (red)
Laser not functioning Contact BD Biosciences.
Optical alignment problem Contact BD Biosciences.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
LSR2.book Page 122 Tuesday, April 25, 2006 3:34 PM
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