196 BD LSR II User’s Guide
5 Set an initial laser delay value ONLY for the laser whose delay is to be
optimized.
• If you are optimizing the violet laser, set its delay to 20 µsec.
• If you are optimizing the UV laser, set its delay to 40 µsec.
• If you are optimizing the red laser, set its delay to 60 µsec.
6 While observing the positive events on the histogram plot, adjust the laser
delay in 1 µsec increments within a range of 10 µsec of the initial setting.
Choose the setting that moves the events farthest to the right (highest
fluorescence intensity).
7 Draw an interval gate on the histogram plot for the positive events.
8 Create a statistics view to display the mean fluorescence intensity of the
gated population.
9 While observing the mean fluorescence intensity for the gated population,
adjust the laser delay in 0.1 µsec increments within a range of 2.0 µsec of
the setting obtained in step 6.
You should also stay within a range of 10 µsec of the initial setting (step 5).
Preserve the setting that maximizes the fluorescence intensity.
10 Reset the window extension to 10 µsec.
LSR2.book Page 196 Tuesday, April 25, 2006 3:34 PM
To Next Page
Loading...
