411829-L, Operation April 2012 Page 4-9
4
5. Specify the collection and fractionation settings to be used in the Parameters field. Refer to
Table 4-2 below for guidance. For more information; see the “Collection and Fractionation”
appendix on page A-1.
Save and/or Run the Method
1. If you want to save the method and reuse it in the future:
a. To save the method in the system’s database, press Save. If your user account is
password-protected, the Password dialog opens. Enter your password, using the keypad,
and press OK.
b. To save the method on a USB memory device or the network:
1. Press Save As. The Save As dialog opens.
2. Select the user name and enter the method name by selecting the corresponding text box.
3. To save the method on a USB memory device, connect the device to the USB port
located underneath the touch screen and press Save.
4. To save the method in a share folder on your network, press Save. (If the button is
disabled, no file sharing has been set up. See “Set Up File Sharing and Auto Save of
Reports” on page 3-7.)
2. To run a purification using the (saved or unsaved) method, see “Prepare and Run a Purification”
on page 4-15.
NOTE
You do not have to save your method to be able to run it.
Table 4-2: Collection and Fractionation Parameters
Parameter Description
Start
Wavelength
The shortest wavelength to be included in the λ-all signal. The range is 200 to 400 nm
(dual variable UV detector) or 200 to 800 nm (UV-VIS detector). Note that this
parameter is only available with the λ-all detection mode.
End Wavelength The longest wavelength to be included in the λ-all signal. The range is 200 to 400 nm
(dual variable UV detector) or 200 to 800 nm (UV-VIS detector). Note that this
parameter is only available with the λ-all detection mode.
Collect All Light absorbance of sample is monitored, but collection is based on the gradient’s total
volume. When using the detection mode UV1+UV2 or UV1, valley fractionation is
enabled by default in the System mode. If disabled, fractionation is based on the max
fraction volume for the vessel type.
Start Threshold Used to collect samples with an absorbance exceeding the set absorbance threshold.
Initial Waste Delay collection until a specified volume has been delivered to the waste.
Slope Collection is based on the start slope of light peaks. Refer to the “Collection and
Fractionation” appendix on page A-1 for details. Note that slope collection is only
possible with the UV1 and UV1+UV2 detection modes.
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