Connect tubing to ÄKTA pure
ActionStep
Lift out the Tubing holder (4) from the Delivery arm (1).1
Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut (5) from the
Tubing holder.
2
Insert the tubing through the Tubing holder.3
Place the Tubing holder with the tubing over the Tube adjustment cavity (2) of the Delivery
arm. Push the tubing down against the bottom of the Tube adjustment cavity, and then
fingertighten the Tubing holder nut. This ensures the correct length of the exposed tubing
end (6).
4
Re-install the tubing holder in the Delivery arm.5
For Fraction collector F9-R: Connect the tubing from the fraction collector to the port
Frac on the outlet valve.
6
For Fraction collector F9-R, 2nd: Connect the tubing from the second fraction collector
to:
•
port Out 10 on Outlet valve V9-O or V9H-O
•
port Out 1 on Outlet valve V9-Os or V9H-Os
Adjust the delay volume setting in UNICORN to the volume of the tubing, see Section 9.8.8
System settings - Tubing and Delay volumes, on page 460 for more details.
7
ÄKTA pure User Manual 29119969 AB 107
3 ÄKTA pure external modules
3.3 Fraction collector F9-R