ActionStep
In the Process Picture:3
•
Click on the buffer inlets.
•
Select the position of the inlet to be filled. Select the positions in reverse alphabetical
order and start with the highest number. For example, if all the four inlets in Inlet valve
AB are to be filled, fill them in the following order: B2, B1, A2, A1.
Result: The inlet valve switches to the selected port.
Connect a 25 to 30 ml syringe to the purge valve of one of the pump heads of the pump
that is being prepared. Make sure that the syringe fits tightly into the purge connector.
4
Open the purge valve by turning it counter-clockwise about three quarters of a turn.
Draw liquid slowly into the syringe until the liquid reaches the pump.
5
Close the purge valve by turning it clockwise. Disconnect the syringe and discard its
contents.
6
Repeat steps 3 to 6 for each piece of inlet tubing that is to be used during the run.7
Purge System pump B
Follow the instruction below to purge both pump heads of System pump B.
ActionStep
Make sure that the piece of waste tubing connected to the Injection valve port W1 is
placed in a waste vessel.
1
ÄKTA pure User Manual 29119969 AB 163
5 Operation
5.4 Prime inlets and purge pump heads
5.4.1 System pumps
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