ActionStep
When buffer leaves the tubing at the bottom of the column in a continuous mode, connect
this piece of tubing to the Column valve. Use the port opposite to the one already con-
nected to the column, in this example port 1B. If no column valve is used, connect the
tubing to the next module in the flow path.
9
Click the End icon in the System Control toolbar to end the run.10
176 ÄKTA pure User Manual 29119969 AB
5 Operation
5.5 Connect a column
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