System storage
If the instrument is not going to be used for a couple of days or longer, also perform the following:
•
Fill the system and inlets with storage solution (e.g., 20% ethanol) using System CIP.
If Column valve V9-C or V9H-C is mounted, the integrated pressure sensor of the valve allows
the system to monitor the post-column pressure. The limit for the pressure sensor in Column
valve V9-C or V9H-C is automatically set so that the UV monitor and the pH monitor are
protected from high pressure. If Column valve V9-C or V9H-C is not mounted, make sure
to keep the pressure in the system after the column below the pressure limits for the modules
in the flow path.
Note:
•
If applicable, prepare the pH electrode for storage as described in Section 7.6.6 Storage of the pH
electrode, on page 291.
Column cleaning
After a method run is completed, perform the following:
•
Clean the column with one or several cleaning solution(s) using Column CIP.
Column storage
If the column is not going to be used for a couple of days or longer, also perform the following:
•
Fill the column with storage solution (e.g., 20% ethanol) using Column CIP.
pH electrode storage
If pH monitoring will not be used for a week or longer, perform one of the following actions:
•
Inject new storage solution into the pH flow cell. Refer to Section 7.6.6 Storage of the pH electrode,
on page 291 for instructions.
•
Replace the pH electrode with the dummy electrode that is installed in the pH valve on delivery.
In the following situations, in order to increase the lifetime of the pH electrode, use the By-pass position
and store the electrode in storage solution inside the pH flow cell:
•
pH monitoring is not needed during the run.
•
Organic solutions are used.
•
Extremely acidic or extremely basic solutions are used.
For further information on how to prepare the pH electrode for storage, refer to Section 7.6.6 Storage
of the pH electrode, on page 291.
222 ÄKTA pure User Manual 29119969 AB
5 Operation
5.9 Create a method and perform a run
5.9.4 After run procedures
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