Assemble the pump head
Make sure to assemble the pump head correctly. Refer to Illustrations, on page 336.
ActionStep
Place the drain plate on top of the assembly. Screw the two screws through the drain
plate and the rinse chamber using a cross-headed screwdriver.
1
Wipe clean the piston and remove all finger prints. Wet the piston with 20% ethanol, and
insert the piston into the return spring. With the pump head facing downwards on the
bench, insert the piston into the pump head by pushing it gently but firmly vertically
downwards into the seal.
2
Place the complete pump head over the locating pins on the front panel of the sample
pump module. Turn the pump head so that the text UP on the drain plate is facing up-
wards. Push firmly on the front of the pump head, and at the same time screw one of
the screws to fasten the pump head onto the front of the module using a hex wrench.
Screw the second screw of the pump head. Make sure to tighten both screws fully.
3
Reconnect the tubing of the pump piston rinsing system.4
Reconnect the inlet manifold.5
Reconnect the tubing to the pump head, and reconnect the pump inlet tubing.6
Break in the new pump piston seal, see instruction below.7
Break in the new pump piston seal
Follow the instruction below to break in the new pump piston seal of pump P9-S.
ActionStep
Fill a buffer vessel with 20% ethanol in water. Immerse a piece of sample inlet tubing, for
example S1, in the buffer vessel. Place the buffer vessel on the Buffer tray.
1
Prime the inlets and purge the pump, see Section 5.4.2 Sample pump, on page 1682
Connect the reference capillary Ref 1 (or equivalent tubing that gives a backpressure of
2-3 MPa) to one of the column positions of the Column valve (e.g., ports 1A and 1B).
3
Immerse the waste tubing in the buffer vessel to recirculate the liquid.4
ÄKTA pure User Manual 29119969 AB 349
7 Maintenance
7.8 Replacement procedures
7.8.11 Replace pump piston seals of Pump P9-S
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