ActionStep
In the Manual instructions dialog:5
•
Select Flow path:Column position, and select the Position of the tubing connected
to the Column valve, in this example 1. Click Insert.
•
Select Flow path:Sample inlet and select a Position, in this example S1. Click Insert.
•
Select Flow path:Injection valve and select Direct inject from the Position drop-down
list. Click Insert.
•
Select Pumps:Sample flow and set the Flow rate to 25.0 ml/min. Click Insert.
•
Click Execute.
Result: A sample flow of 25 ml/min starts.
Run the flow for 2 hours.6
Discard the used buffer.7
350 ÄKTA pure User Manual 29119969 AB
7 Maintenance
7.8 Replacement procedures
7.8.11 Replace pump piston seals of Pump P9-S