Possible cause and actionProblem
•
Wrong wavelength for current buffer
Change wavelength or buffer.
•
Dirt in the UV flow cell
Clean the UV cell. See Section 7.5.1 Clean the UV flow cell, on
page 273.
•
Unclean optical fiber connectors
Clean the connectors. See Section 7.8.5 Replace the UV monitor
U9-M flow cell, on page 325.
•
Broken UV flow cell
Replace UV flow cell. See Section 7.8.5 Replace the UV monitor
U9-M flow cell, on page 325.
No light transmission
through the UV cell
•
Wrong wavelength for current buffer
Change wavelength or buffer.
•
Unclean optical fiber connectors
Clean the connectors. See Section 7.8.5 Replace the UV monitor
U9-M flow cell, on page 325.
Autozero out of accepted
range
•
The air intake on the rear or on the left side of the instrument
is covered
Make sure that none of the air intakes on the instrument are
covered.
•
Hot surroundings
Decrease the room temperature. Maximum operating tempera-
ture is 35°C.
•
Hardware error
Switch off the instrument and wait until the temperature has
decreased. Restart the instrument. If this error is recurrent, gen-
erate a System error report and contact Service.
The internal temperature of
the UV monitor is too high
•
No UV flow cell is attached
Attach UV cell. See Section 7.8.5 Replace the UV monitor U9-M
flow cell, on page 325.
•
UV flow cell is not correctly installed.
Verify that the UV cell is correctly installed. See Section 7.8.5 Re-
place the UV monitor U9-M flow cell, on page 325
•
The UV flow cell is broken
Replace the cell. See Section 7.8.5 Replace the UV monitor U9-M
flow cell, on page 325.
UV cell path length unread-
able
ÄKTA pure User Manual 29119969 AB 361
8 Troubleshooting
8.3 Troubleshooting: Monitors
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