GE AKTApure - Page 362

Possible cause and actionProblem
•
Air in the UV flow cell
Use the Flow restrictor. Use the pH valve instruction to manually
set the Flow restrictor inline (Flow path:pH valve:Restrictor in-
line), or select the Flow restrictor in the Method Settings phase
of a method.
Remove the air by flushing the cell with water or buffer. If persis-
tent, clean the UV cell. See Section 7.5.1 Clean the UV flow cell,
on page 273.
•
Air in buffers
De-gas if necessary.
•
Dirt in the UV flow cell
Clean the UV cell. See Section 7.5.1 Clean the UV flow cell, on
page 273.
•
Dirt in the flow path
Clean the system in accordance to Section 7.6.2 Perform System
CIP, on page 278. Clean the column in accordance to Section 7.6.3
Perform Column CIP, on page 284.
Ghost peaks
362 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.3 Troubleshooting: Monitors