Possible cause and actionProblem
•
Bad pump function
Check that the pump is operating properly. See Examples of pump
pressure curves, on page390 for example of pump pressure curves.
•
Air in the flow path
Purge the pumps. See Section 5.4.1 System pumps, on page 162.
•
Poor mixing function
Check that the correct Mixer chamber size is used. See Select
Mixer chamber, on page152 for recommendations. To change the
Mixer, see Section 7.8.3 Replace the Mixer, on page 322.
Check the motor operation of the Mixer. Place a magnet close to
the mixer chamber during run. The magnet should vibrate.
Check that the Mixer chamber is free from solids. To open the
mixer, see Section 7.3.2 Replace the inline filter, on page 266.
Waves on the gradient
•
A charged particle has been detected
Prepare the sample so that charged particles are eliminated.
•
Air bubbles are passing through the flow cell
Check for loose tubing connections.
Use the Flow restrictor.
Ghost peaks appear in the
gradient profile
•
Dirt in the tubing
Make sure that the tubing has been washed properly.
•
Bad pump function
Make sure that the pump operates properly. See Examples of
pump pressure curves, on page390 for example of pump pressure
curves.
•
The Mixer chamber is too large
Change to a Mixer chamber with a smaller volume. See Select
Mixer chamber, on page152 for recommendations. To change the
Mixer, see Section 7.8.3 Replace the Mixer, on page 322.
Non-linear gradients or
slow response to %B
changes
ÄKTA pure User Manual 29119969 AB 369
8 Troubleshooting
8.3 Troubleshooting: Monitors
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