Possible cause and actionProblem
The Fraction collector wash instruction is aborted when the fraction
collector door is opened during state pause. When selecting to
continue the run the instruction is reported as Completed, it should
state Cancelled.
Fraction collector wash is
reported as Completed
when it has been aborted.
Action: Do not open the door during the Fraction collector wash
instruction. If the door has been opened, restart the Fraction collec-
tor wash instruction to make sure that the Fraction collector wash
has been performed as recommended.
•
The Detector - Outlet valve delay volume has been set to zero.
1
In System Control, select System:Settings:Fraction collec-
tions:Delay volumes.
2
Correct the Detector - Outlet valve delay volume.
The instrument does not
fractionate via the Outlet
valve ports
Action: End a manual run before starting a method run.When starting a method run
during a manual run, the
fraction collector generates
an error message indicating
that the wrong Cassettes
are in place, even though
the correct Cassettes are
present in the Fraction col-
lector.
Action:High pressure alarm when
collecting fractions with the
fraction collector
•
Decrease the flow rate or use Outlet valve fractionation,
or
•
replace the following tubing with tubing of larger inner diameter
(i.d.)
-
the tubing between the outlet valve and the fraction collector
and
-
the internal tubing of the fraction collector.
Note:
Update the delay volume. In the System Control software module,
select System:Settings:Tubing and Delay volumes and update
the value for Delay volume: Monitor to frac.
Action: If possible, place the Cassettes close to waste, i.e., position
5 or 6 during fractionation.
Spillage in Fraction collector
when the Frac arm is mov-
ing from Cassette placed in
position 1 and 2 to waste
384 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.5 Troubleshooting: Fraction collector
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