Possible cause and actionProblem
•
Unclean diode windows in the Drop sync sensor or unclean
nozzle
Clean the Drop sync diode windows and the nozzle. See Sec-
tion 7.6.4 Clean Fraction collector F9-C, on page 288. Use the
Fraction collector cleaning position.
•
Too high flow rate during usage of Drop sync
Use a flow rate below 2 ml/min.
•
One or more Cassettes have empty positions
Make sure that all Cassette positions contain tubes or plates.
•
The tubes are flooded
Make sure that the fraction volume is adapted to the tube volume.
Fraction tubes have not been replaced by empty tubes when the
Fraction collector was opened.
Spillage by the fraction col-
lector during fractionation
•
The door of the fraction collector is not properly closed
Close the door.
It is not possible to fraction-
ate
•
Quick scan has not detected the correct Cassette
Clean the Cassette type code of the Cassette. See Section 7.6.4
Clean Fraction collector F9-C, on page 288.
•
Unsupported deep well plate is used.
Make sure that approved deep well plates are used. See Deep
well plates, on page 101.
•
Wells in deep well plates are prefilled to a volume above 25%
of the total well volume
Full scan will not work with prefilled wells during these conditions.
•
The deep well plate is not correctly positioned in the Cassette
See Prepare and insert the Cassette tray, on page195 for informa-
tion of how to place the deep well plate.
•
Dirt on the nozzle or Drop sync sensor diode windows. Dirt
may effect where the drops fall.
Clean nozzle and Drop sync sensor diode windows. See Sec-
tion 7.6.4 Clean Fraction collector F9-C, on page 288.
The fraction collector frac-
tionates in the wrong well
or tube
•
Some parts in a method require that the door of the fraction
collector is closed.
Do not open the door when the Fractionation indicator is lit.
Error message when the
door of the fraction collec-
tor is opened during a run
ÄKTA pure User Manual 29119969 AB 383
8 Troubleshooting
8.5 Troubleshooting: Fraction collector
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