Possible cause and actionProblem
•
The waste tubing is positioned so that the flow is obstructed
Untwist the waste tubing.
•
The waste container is placed in a position higher than the
waste outlet
Place the waste container in a position lower than the waste
outlet.
•
The waste tubing is blocked.
Clean or replace the waste tubing.
The waste or the interior of
the fraction collector is
flooded
•
Check the position of the waste funnel
Refit the waste funnel.
The liquid leaving the nozzle
does not strike the waste
funnel
•
Salt residuals in the tubing
Perform a Fraction collector wash, see Section 7.6.4 Clean
Fraction collector F9-C, on page 288.
If persistent, contact Service.
The tubing in the fraction
collector is blocked
•
Leakage on the wet side of the instrument
Localize the leakage and take care of the leakage, for example
by tightening connectors.
•
Air in pumps
Purge pumps. See Section 5.4.1 System pumps, on page 162.
•
Bad pump function
See troubleshooting of pumps in Section 8.6.
•
Leakage inside the Frac chamber
Contact Service.
The fraction volume found
in the tubes or wells are
smaller than expected
•
The fingertight connector on the Dispenser head is not tight
enough
Tighten the connector. Replace the connector if the leakage is
persistent.
•
The waste tubing or the waste funnel is blocked.
Make sure that there is no blockage or clogging and that the
waste tubing not is bent.
•
Leakage in the Frac arm
Contact service.
Liquid on the floor of the
fraction collector
382 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.5 Troubleshooting: Fraction collector
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