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1374.2.5 Configuration of Versatile valves ..................................................................................................................................................................

1384.2.6 Configuration of pH valves .............................................................................................................................................................................

1394.2.7 Configuration of outlet valves .......................................................................................................................................................................

1404.2.8 Configuration of UV monitors ........................................................................................................................................................................

1414.2.9 Configuration of Conductivity monitor ......................................................................................................................................................

1424.2.10 Configuration of external air sensors .........................................................................................................................................................

1434.2.11 Configuration of fraction collectors ............................................................................................................................................................

1454.2.12 Configuration of I/O-box ..................................................................................................................................................................................

1464.3 General system settings ..................................................................................................................................................

1495 Operation ..........................................................................................................................................................

1505.1 Before you prepare the system ....................................................................................................................................

1515.2 Prepare the flow path ........................................................................................................................................................

1585.3 Start UNICORN and connect to system ....................................................................................................................

1615.4 Prime inlets and purge pump heads ..........................................................................................................................

1625.4.1 System pumps ......................................................................................................................................................................................................

1685.4.2 Sample pump .......................................................................................................................................................................................................

1735.5 Connect a column ...............................................................................................................................................................

1775.6 Pressure alarms ...................................................................................................................................................................

1805.7 Sample application .............................................................................................................................................................

1815.7.1 Sample application using direct injection onto the column .............................................................................................................

1835.7.2 Sample application using a Superloop

™

...................................................................................................................................................

1905.7.3 Sample application using a sample loop .................................................................................................................................................

1935.8 Fractionation .........................................................................................................................................................................

1945.8.1 Prepare Fraction collector F9-C ....................................................................................................................................................................

2005.8.2 Prepare Fraction collector F9-R ....................................................................................................................................................................

2075.8.3 Fractionation overview .....................................................................................................................................................................................

2115.9 Create a method and perform a run .........................................................................................................................

2125.9.1 Create a method ..................................................................................................................................................................................................

2175.9.2 Prepare and perform a run .............................................................................................................................................................................

2195.9.3 Monitor a run ........................................................................................................................................................................................................

2215.9.4 After run procedures .........................................................................................................................................................................................

2256 Performance tests ...........................................................................................................................................

2266.1 General performance test actions ..............................................................................................................................

2296.2 Air sensor A and Inlet valve A tests .............................................................................................................................

2316.3 Air sensor B and Inlet valve B tests .............................................................................................................................

2336.4 Air sensor S and Sample inlet valve tests ................................................................................................................

2356.5 Column valve C tests .........................................................................................................................................................

2376.6 Fraction collector F9-C test ............................................................................................................................................

2406.7 Fraction Collector F9-R Test ...........................................................................................................................................

2426.8 Sample pump tests ............................................................................................................................................................

2446.9 System Test UV U9-L (fixed) ............................................................................................................................................

2486.10 System Test UV U9-M (variable) ....................................................................................................................................

2527 Maintenance .....................................................................................................................................................

2537.1 Maintenance Manager .....................................................................................................................................................

2587.2 Maintenance program ......................................................................................................................................................

2617.3 Weekly maintenance .........................................................................................................................................................

2627.3.1 Change pump rinsing solution ......................................................................................................................................................................

2667.3.2 Replace the inline filter .....................................................................................................................................................................................

2677.3.3 Clean the Fraction collector F9-C sensors ................................................................................................................................................

2697.4 Monthly maintenance .......................................................................................................................................................

4 ÄKTA pure User Manual 29119969 AB

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