Solubilization and denaturing agents
The following chemicals are suitable for continuous use, as additives in separation and purification
methods.
CAS no/EC noConcentrationChemical
50-01-1/ 200-002-36 MGuanidinium hydrochloride
151-21-3/ 205-788-11%Sodium dodecyl sulfate (SDS)
9005-64-5/ 500-018-31%Tween™ 20
57-13-6/ 200-315-58 MUrea
Chemicals used in reversed phase
chromatography (RPC)
The following chemicals are suitable for continuous use.
CAS no/EC noConcentrationChemical
75-05-8/ 200-835-2100%Acetonitrile
1
109-99-9/ 203-726-885%/15%Acetonitrile/Tetrahydrofuran
1
N/AMax 0.2% TFAAcetonitrile/water/Trifluoroacetic
acid (TFA)
2
75-08-1/ 200-837-3100%Ethanol
67-63-0/ 200-661-7100%Isopropanol
74-93-1/ 200-659-6100%Methanol
N/AMax 5% formic
acid
Water/organic mobile
phase/formic acid
1
Organic solvents can penetrate weaknesses in PEEK tubing walls more easily than water based buffers. Special
care should therefore be taken with prolonged use of organic solvents close to pressure limits.
Depending on pressure, tubing between pump head and pressure monitor needs to be changed. See Section 7.8.1
Replace tubing and connectors, on page 317 for more information.
2
Mobile phase system.
It is recommended to replace the mixer sealing ring with the highly resistant O-ring (product
code 29-0113-26) if the system is to be exposed to organic solvents or high concentrations of
organic acids, such as acetic acid and formic acid, for a longer period of time.
Note:
434 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.5 Chemical resistance guide
9.5.2 Chemical resistance specifications
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