DescriptionInstruction name
Column packing flow is used to set flow rates over 25 ml/min and
150 ml/min for ÄKTA pure 25 and ÄKTA pure 150, respectively.
Both A and B pumps are used to generate the flow, making it
possible to set flow rates up to 50 ml/min and 300 ml/min for
ÄKTA pure 25 and ÄKTA pure 150, respectively. Flow rate can be
set either as volumetric or as linear flow. A column type must be
selected before using linear flow.
Column packing flow
Before executing the Column packing flow instruction it is impor-
tant to:
•
Immerse inlet tubing A1 and B1 in the same buffer
•
Disconnect the column outlet tubing from the Column valve
and place the tubing in a waste vessel
Note:
When running Column packing flow only isocratic runs can be
performed, gradients cannot be generated.
Delay volume: Monitor to outlet valve is used to define the delay
volume between the monitor and the Outlet valve. The instruction
is used to make sure that the collected fractions correspond to
the fractions indicated in the chromatogram. The delay volume
must be changed when changing tubing to another i.d. or length
or when removing or adding components.
Delay volume: Monitor to outlet
valve
Delay volume: Monitor to frac is used to define the delay volume
between the monitor and the Fraction collector. The instruction
is used to make sure that the collected fractions correspond to
the fractions indicated in the chromatogram. The instruction is
available only when the Fraction collector is selected in the com-
ponent list.
Delay volume: Monitor to frac
Delay volume: Monitor to frac 2 is used to define the delay volume
between the monitor and the second Fraction collector. The in-
struction is used to make sure that the collected fractions corre-
spond to the fractions indicated in the chromatogram. The instruc-
tion is available only when the second Fraction collector is selected
in the component list.
Delay volume: Monitor to frac 2
Start volume count starts the volume counter function. The
counted volume is saved into a memory.
Start volume count
This instruction is best used in combination with Watch instruc-
tions.
ÄKTA pure User Manual 29119969 AB 485
9 Reference information
9.9 Manual instructions
9.9.9 Manual instructions - Advanced
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