IMPORTANT
Before you mount the U-FFWR make sure you insert all excitation filters into
the filter wheel. Note the position of the individual filters as you might need
them later for the observation method adjustment. If you mount them as
set out in the table below, less adjustments will have to be made later.
U-FFWR position 25 mm excitation filter
1 DAPI
2 FITC
3 CY3
4 CY5
5 CY7
6 Black-out filter
7 Black-out filter
8 Black-out filter
Refer to the U-FFWR instruction manual to the chapter for filter insertion for more
information.
The orientation for Semrock emission filters is with the arrow towards the
camera.
The orientation for Chroma emission filters is with the arrow pointing away
from the camera.
1. Attach the U-FFWR to the flange of the IX3-RFALFE at the back of the sys-
tem and tighten the two 3 mm headless hex screws.
- 46 -
Instructions for use
11 Mounting fluorescence components
OLYMPUS VS200 System
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