Procleix TIGRIS - Contents

CONTENTS
vi PROCLEIX
®
TIGRIS System Operator’s Manual
Part # 902649EN Rev. A Volume I: Operating the Instrument
Overview of the PROCLEIX
®
Assays on the PROCLEIX
®
TIGRIS System ....... 1-18
Target Capture........................................................................................... 1-19
Transcription-Mediated Amplification (TMA).............................................. 1-19
Hybridization Protection Assay (HPA) ....................................................... 1-20
Dual Kinetic Assay (DKA) .......................................................................... 1-20
Specifications ...................................................................................................... 1-21
Physical Specifications .............................................................................. 1-21
Environment............................................................................................... 1-21
Water for the PROCLEIX
®
TIGRIS System Specifications ....................... 1-22
Power Requirements ................................................................................. 1-22
Vacuum Pressure Requirements............................................................... 1-22
Sample Tube Specifications ...................................................................... 1-22
Sample Requirements ............................................................................... 1-25
System Capacities and Throughput........................................................... 1-26
Operator Prerequisites ........................................................................................ 1-26
Glove Changing Recommendations.................................................................... 1-27
SECTION 2. SETTING UP THE INSTRUMENT ............................................................2-1
Preparing the Workspace...................................................................................... 2-1
Starting the System ............................................................................................... 2-1
Monitoring System Status ..................................................................................... 2-2
System Status and Inventory Status Panels................................................ 2-2
Return Times Panel ..................................................................................... 2-3
Status Bar .................................................................................................... 2-4
Managing Inventories ............................................................................................ 2-6
Loading Consumables........................................................................................... 2-6
Loading Pipette Tips .................................................................................... 2-6
Loading Multi-Tube Units (MTUs)................................................................ 2-7
Preparing the Lower Bay....................................................................................... 2-9
Replacing Depleted Fluids......................................................................... 2-10
Scanning Fluid Barcodes........................................................................... 2-14
Emptying Waste Containers ...................................................................... 2-15
Validating the Lower Bay Inventory ........................................................... 2-19
Priming the System.................................................................................... 2-19
Loading Assay Reagents .................................................................................... 2-20
Working with Master Lots .......................................................................... 2-20
Loading ARs .............................................................................................. 2-22
Changing the Reagent Quadrant............................................................... 2-24