Thermo NanoDrop One - Page 17

2
Applications
Detection Limits for All Applications
Thermo Scientific NanoDrop One User Guide 11
Detection limits for pre-defined dyes
Sample Type Lower Detection Limit Upper Detection Limit
a
a
Values are approximate
Typical Reproducibility
b
b
Based on five replicates (SD=ng/μL; CV=%)
Cy3, Cy3.5, Alexa Fluor
555, Alexa Fluor 660
0.2 pmol/μL (pedestal) 100 pmol/μL (pedestal) ±0.20 pmol/μL for sample
concentrations between 0.20 and 4.0
pmol/μL;
±2% for samples >4.0 pmol/μL
Cy5, Cy5.5, Alexa Fluor
647
0.12 pmol/μL (pedestal) 60 pmol/μL (pedestal) ±0.12 pmol/μL for sample concentrations
between 0.12 and 2.4 pmol/μL;
±2% for samples >2.4 pmol/μL
Alexa Fluor 488, Alexa
Fluor 594
0.4 pmol/μL (pedestal) 215 pmol/μL (pedestal) ±0.40 pmol/μL for sample concentrations
between 0.40 and 8.0 pmol/μL;
±2% for samples >8.0 pmol/μL
Alexa Fluor 546 0.3 pmol/μL (pedestal) 145 pmol/μL (pedestal) ±0.30 pmol/μL for sample concentrations
between 0.30 and 6.0 pmol/μL;
±2% for samples >6.0 pmol/μL