Thermo NanoDrop One User Manual

Measure Protein A205
84 NanoDrop One User Guide Thermo Scientific
Related Topics
• Instrument Settings
Setting Available Options
Mass Ext. Coefficient
(L/gm-cm)
Description
Sample type 31 31 Assumes 0.1% (1 mg/mL) at 205 nm = 31
Scopes 27 + 120 * (A280/A205) Assumes
0.1% (1 mg/mL) at 205 nm = 27 + 120 *
(A280/A205)
Other protein
(
1%)
User entered mass
extinction coefficient
Assumes protein has known mass extinction coefficient
(). Enter mass extinction coefficient in L/gm-cm for
1mg/mL (
0.1%) protein solution.
Baseline
Correction
On or off
Enter baseline correction
wavelength in nm or use
default value (340 nm)
N/A Corrects for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from
absorbance values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.
Tip: If the sample has a modification that absorbs light
at 340 nm, select a different correction wavelength or
turn off Baseline Correction.

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)