Thermo NanoDrop One - Page 90

Measure Protein A205
84 NanoDrop One User Guide Thermo Scientific
Related Topics
• Instrument Settings
Setting Available Options
Mass Ext. Coefficient
(L/gm-cm)
Description
Sample type 31 31 Assumes 0.1% (1 mg/mL) at 205 nm = 31
Scopes 27 + 120 * (A280/A205) Assumes
0.1% (1 mg/mL) at 205 nm = 27 + 120 *
(A280/A205)
Other protein
(
1%)
User entered mass
extinction coefficient
Assumes protein has known mass extinction coefficient
(). Enter mass extinction coefficient in L/gm-cm for
1mg/mL (
0.1%) protein solution.
Baseline
Correction
On or off
Enter baseline correction
wavelength in nm or use
default value (340 nm)
N/A Corrects for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from
absorbance values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.
Tip: If the sample has a modification that absorbs light
at 340 nm, select a different correction wavelength or
turn off Baseline Correction.