Measure Protein A205
86 NanoDrop One User Guide Thermo Scientific
Related Topics
• Beer-Lambert Equation
• Protein A280 Calculations
Sample Pathlength
• For micro-volume measurements, the software selects the optimal
pathlength (between 1.0 mm and 0.03 mm) based on sample
absorbance at the analysis wavelength.
• For cuvette measurements, pathlength is determined by the cuvette
Pathlength setting in the software (see General Settings).
• Displayed spectra and absorbance values are normalized to a 10 mm
pathlength equivalent.
Reported Values
• Protein concentration. Reported in selected unit (mg/mL or μg/mL).
Calculations are based on Beer-Lambert equation using corrected
protein absorbance value.
To Next Page
Loading...
