BD FACSymphony A5 SE

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14. Adjust the red laser area scaling factor until the APC-A signal matches the APC-H signal, if needed.

Chapter 8 Manual settings 101

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Bd Facsymphony™ A5 Se1
Table of Contents3
About this Guide7
What this Guide Covers8
Conventions8
About the Documentation9
Instrument Technical Support10
Introduction11
System Overview12
Cytometer Overview12
Control Panel14
Fluidics System15
Sheath and Waste Containers19
Optics20
Workstation22
Cytometer Setup23
Starting the Cytometer and Computer24
Preparing the Sheath Container25
Removing Air Bubbles27
Preparing the Waste Container29
Preparing the Fluidics31
Adding a Bead Lot Number32
Custom Configurations and Baselines34
Maintenance35
Maintenance Overview36
Cleaning the Fluidics37
Shutting down the Cytometer38
Flushing the System38
Replacing the Waste Container Cap40
Changing the Sheath Filter41
Changing the Bal Seal42
Changing the Sample Tube O-Ring44
Cleaning or Replacing the Sheath Gasket45
Spectral Functions47
Spectral Function Overview48
Autofluorescence54
Spectral Unmixing Software Considerations55
Setting up a New Spectral Experiment57
Creating a Spectral Unmixing Matrix59
Recording and Analyzing Spectral Data62
Repeating a Spectral Experiment65
Optimizing Cytometer Settings67
Cytometer Settings Workflow68
Verifying the Configuration and User Preferences69
Running a Performance Check71
Setting up a Compensation Experiment74
Creating Application Settings77
Recording Compensation Controls78
Calculating Compensation81
Recording and Analyzing Data83
Data Recording and Analysis Workflow84
Preparing the Workspace84
Recording Data85
Analyzing Data87
Reusing an Analysis91
Manual Settings93
About Laser Delay94
Optimizing Laser Delay95
Adjusting Area Scaling97
Troubleshooting103
Cytometer Troubleshooting104
Electronics Troubleshooting109
Supplies and Consumables111
Ordering Information112
Beads112
Reagents113

BD FACSymphony A5 SE - Page 101

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