BD FACSymphony A5 SE - Page 89

l
In the FITC vs PE plot, draw a gate around the PE-positive population. Name the population PE positive in
the population hierarchy.
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In the FITC vs PerCP-Cy5.5 plot, draw a gate around the PerCP-Cy5.5-positive population. Name the
population PerCP-Cy5.5 positive in the population hierarchy.
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In the FITC vs APC plot, draw a gate around the APC-positive population. Name the population APC
positive in the population hierarchy.
11. Format the statistics view.
a. Right-click the statistics view and select Edit Statistics View.
b. Click the Header tab and select the Specimen Name and Tube Name checkboxes.
c. Click the Populations tab and select all populations except All Events. Clear the %Parent, %Total, and
#Events checkboxes.
d. Click the Statistics tab and select the mean for each of the fluorescence parameters.
e. Click OK.
Chapter 7 Recording and analyzing data 89