92 BD LSR II User’s Guide
• Within the Ratio tab, click the Add button. Select Indo-1 (Violet)-A for
the Numerator, and Indo-1 (Blue)-A for the Denominator.
6 Create a new specimen in the CalciumFlux experiment.
7 Open the specimen to access its tube. Rename the tube CaF_001, and move
the current tube pointer to CaF_001.
At this point, your experiment should look similar to this one.
8 Create a normal worksheet for the CaF_001 tube with the following dot
plots:
• FSC-A vs SSC-A
• Indo-1 (Blue)-A vs Indo-1 (Violet)-A
• Time vs Ratio: Indo-1 (Violet)-A/Indo-1 (Blue)-A
LSR2.book Page 92 Tuesday, April 25, 2006 3:34 PM
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